基质和外源激素处理对多花勾儿茶高空压条生根的影响

doi:10.13466/j.cnki.lczyyj.2024.02.010

林草资源研究 ›› 2024›› Issue (2): 80-91.doi: 10.13466/j.cnki.lczyyj.2024.02.010

基质和外源激素处理对多花勾儿茶高空压条生根的影响

赵恬¹(), 王胤晨¹, 周思旋¹, 曾兵³, 袁扬¹, 邓梦青¹, 廖艳¹, 张锦华³()

1.贵州省农业科学院畜牧兽医研究所,贵阳 550002
2.贵州省农业科学院草业研究所,贵阳 550025
3.西南大学动物科学技术学院,重庆 402460

收稿日期:2023-12-26 修回日期:2024-01-18 出版日期:2024-04-28 发布日期:2024-09-02
通讯作者: 张锦华,研究员,博士,主要从事蜜源植物种质资源、牧草种质资源研究。Email:420506337@qq.com
作者简介:赵恬,助理研究员,硕士,主要从事蜜源植物种质资源研究。Email:zhaotianyeah@163.com
基金资助:
贵州省科技计划项目“贵州药蜜型植物勾儿茶属种质资源鉴选和高效繁育技术研究与示范”(黔科合支撑[2021]一般226);国家自然科学基金“勾儿茶属植物内生真菌天然活性物质分离鉴定及抗菌效应研究”(31960716);贵阳市科技计划项目“林下中蜂高效养殖关键技术研究与推广示范”(筑科合同[2023]2-6号)

Effect of Substrates and Exogenous Hormone on the Rooting of Air Layering of Berchemia floribunda(Wall.)Brongn.

ZHAO Tian¹(), WANG Yinchen¹, ZHOU Sixuan¹, ZENG Bing³, YUAN Yang¹, DENG Mengqing¹, LIAO Yan¹, ZHANG Jinhua³()

1. Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang 550002,China
2. Institute of Prataculture,Academy of Agricultural Sciences,Guiyang 550025,China
3. College of Animal Science and Technology,Southwest University,Chongqing 402460,China

Received:2023-12-26 Revised:2024-01-18 Online:2024-04-28 Published:2024-09-02

摘要/Abstract

摘要：

多花勾儿茶是我国集蜜源、观赏和药用等功能为一体的传统珍贵树种,具有较好的生态和经济效益。为优选其高空压条繁殖的最佳条件,提高和完善其无性快繁技术,采用四因素三水平L₉(3⁴)正交试验设计,探究不同基质配比、外源激素种类、外源激素浓度以及环剥宽度对多花勾儿茶高空压条生根的影响,并运用模糊数学隶属函数法和相关性分析法对各项生根指标进行综合评价,探讨4种因素对压条生根的作用机制。结果显示,适宜的基质和外源激素配比可显著促进多花勾儿茶形成愈伤组织并生根,同时可使各项根部指标增加。方差分析和极差分析表明,生根效果存在基质、外源激素和环剥的差异,其中基质配比和外源激素种类是影响各生根指标的最主要因素。综合评价得出,最有利于多花勾儿茶压条生根的条件为:珍珠岩+草炭+蛭石(1∶1∶1)作为基质、NAA作为生长调节剂、NAA浓度为400 mg/L,环剥宽度为10 mm,该条件下愈伤组织诱导率高达(93.33±2.36)%,生根率高达(85.00±4.08)%,(9.67±0.47)d即可在伤口处形成愈伤组织,(14.00±0.82)d开始生根,平均一级根数[(15.44±0.51)根]、平均一级根长[(7.13±0.25)cm]、平均一级根粗[(3.03±0.21)mm]、根鲜质量[(3.29±0.05)g]、根干质量[(0.67±0.04)g]、根系效果指数(5.50±0.06)、平均隶属函数值(0.77)均为最高。该研究可为建立多花勾儿茶快繁体系,推广多花勾儿茶栽培提供理论参考和数据支撑。

关键词: 多花勾儿茶, 高空压条, 生根, 基质, 外源激素, 正交试验

Abstract:

Berchemia floribunda(Wall.)Brongn.is a traditional precious tree species in China with functions as a nectar source,ornamental and medicinal source,and has good ecological and economic benefits.In order to optimize the optimal conditions for B.floribunda air layering propagation and to improve the vegetative propagation technology,the L₉(3⁴)orthogonal matrix experiment was performed to explore the effects of different substrate ratios,exogenous hormone types,exogenous hormone concentration and girdle width on rooting of its air layering,subordination function method and correlation analysis method were used to comprehensively evaluate various rooting indices,and the mechanism of these four factors on rooting explored.The results showed that a suitable substrate and exogenous hormone ratio can promote the formation of callus,along with an increase in various rooting indices.Variance analysis and range analysis indicated that the efficiency of air layering was affected by the substrate ratio,exogenous hormone and girdle width,substrates ratio and exogenous hormone types were the two most important factors.Based on a comprehensive evaluation and correlation analysis,it is concluded that the most favorable treatment condition for B.floribunda rooting is:perlite+peat+vermiculite(1∶1∶1)as substrates,NAA is used as an exogenous hormone types with a concentration of 400 mg/L,and the girdle width is 10 mm.Under this treatment,the callus induction rate is as high as (93.33±2.36)%,and the rooting rate is as high as(85.00±4.08)%,(9.67±0.47)days to form a callus at the wound site,(14.00±0.82)days start to take root,average number of first order roots[(15.44±0.51)roots],average length of first order roots[(7.13±0.25)cm],average diameter of first order roots[(3.03±0.21)mm],fresh weight[(3.29±0.05)g],dry weight[(0.67±0.04)g],root effect index(5.50±0.06),average subordinate function value(0.77)were the highest.The results of this study provide theoretical references and data support for establishing a rapid propagation system of B.floribunda and promoting its cultivation.

Key words: Berchemia floribunda(Wall.)Brongn., air layering, rooting, substrates, exogenous hormone, orthogonal experiment

中图分类号:

S897

赵恬, 王胤晨, 周思旋, 曾兵, 袁扬, 邓梦青, 廖艳, 张锦华. 基质和外源激素处理对多花勾儿茶高空压条生根的影响[J]. 林草资源研究, 2024,(2): 80-91.

ZHAO Tian, WANG Yinchen, ZHOU Sixuan, ZENG Bing, YUAN Yang, DENG Mengqing, LIAO Yan, ZHANG Jinhua. Effect of Substrates and Exogenous Hormone on the Rooting of Air Layering of Berchemia floribunda(Wall.)Brongn.[J]. Forest and Grassland Resources Research, 2024,(2): 80-91.

图/表 11

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水平	因素
水平	基质配比 (A)	外源激素种类 (B)	外源激素浓度(C)/ (mg/L)	环剥宽度 (D)/ mm
1	草炭土+珍珠岩 (2∶1)	ABT 1	200	10
2	草炭+珍珠岩+蛭石 (3∶1∶1)	IBA	400	14
3	草炭土+珍珠岩+蛭石 (1∶1∶1)	NAA	600	18

处理	因素				因素水平组合
处理	基质配比 (A)	外源激素种类(B)	外源激素浓度(C)	环剥宽度 (D)	因素水平组合
T1	1	1	1	1	A₁B₁C₁D₁
T2	1	2	2	2	A₁B₂C₂D₂
T3	1	3	3	3	A₁B₃C₃D₃
T4	2	1	2	3	A₂B₁C₂D₃
T5	2	2	3	1	A₂B₂C₃D₁
T6	2	3	1	2	A₂B₃C₁D₂
T7	3	1	3	2	A₃B₁C₃D₂
T8	3	2	1	3	A₃B₂C₁D₃
T9	3	3	2	1	A₃B₃C₂D₁
CK

因素	自由度	愈伤组织诱导率		生根率		愈伤组织诱导天数		生根天数
因素	自由度	F	P	F	P	F	P	F	P
A	2	75.46	<0.001	54.86	<0.001	40.53	<0.001	64.26	<0.001
B	2	21.04	<0.001	18.03	<0.001	17.47	<0.001	27.00	<0.001
C	2	7.11	0.005	4.28	0.030	1.34	0.286	1.88	0.181
D	2	3.25	0.062	1.79	0.195	6.59	0.007	9.00	0.002
校正模型	8	26.71	<0.001	19.74	<0.001	16.48	<0.001	25.54	<0.001
R²		0.922		0.898		0.880		0.919

处理	平均一级根数/根	平均一级根长/cm	平均一级根粗/mm	根鲜质量/g	根干质量/g	根系效果指数
T1	8.33±0.34d	4.07±0.25e	1.87±0.06d	1.44±0.15d	0.29±0.01d	1.70±0.17e
T2	8.11±0.51d	4.03±0.15e	1.77±0.06d	1.59±0.49d	0.35±0.04c	1.64±0.17e
T3	7.89±0.51d	4.36±0.21de	1.70±0.10d	2.12±0.11c	0.41±0.05bc	1.72±0.06e
T4	9.11±0.38cd	4.57±0.12de	1.83±0.06d	2.10±0.08c	0.40±0.03c	2.08±0.12de
T5	10.22±0.51c	4.77±0.21d	2.07±0.06cd	2.47±0.10bc	0.45±0.02bc	2.44±0.16d
T6	11.33±0.67bc	5.50±0.20c	2.30±0.10c	2.24±0.10c	0.41±0.04bc	3.11±0.16c
T7	11.45±0.39bc	6.07±0.15bc	2.57±0.12b	2.47±0.12bc	0.44±0.04bc	3.47±0.20bc
T8	12.33±0.67b	6.37±0.21b	2.63±0.06b	2.79±0.20b	0.51±0.03b	3.93±0.34b
T9	15.44±0.51a	7.13±0.25a	3.03±0.21a	3.29±0.05a	0.67±0.04a	5.50±0.06a
CK	4.89±0.69e	3.10±0.26f	1.57±0.06d	1.21±0.16e	0.24±0.02e	0.76±0.13f

因素	自由度	平均一级根数		平均一级根长		平均一级根粗		根鲜质量		根干质量		根系效果指数
因素	自由度	F	P	F	P	F	P	F	P	F	P	F	P
A	2	215.79	<0.001	378.68	<0.001	196.68	<0.001	271.94	<0.001	71.21	<0.001	514.69	<0.001
B	2	33.78	<0.001	45.68	<0.001	16.87	<0.001	64.05	<0.001	28.75	<0.001	82.88	<0.001
C	2	10.36	<0.001	2.81	0.086	4.77	0.22	9.67	0.001	8.79	0.002	21.43	<0.001
D	2	21.84	<0.001	1.76	0.200	18.03	<0.001	21.82	<0.001	9.70	0.001	31.14	<0.001
校正模型	8	18.23	<0.001	107.24	<0.001	59.09	<0.001	91.87	<0.001	29.61	<0.001	162.53	<0.001
R²		0.969		0.979		0.963		0.976		0.929		0.986

基质和外源激素处理对多花勾儿茶高空压条生根的影响

Effect of Substrates and Exogenous Hormone on the Rooting of Air Layering of Berchemia floribunda(Wall.)Brongn.

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处理	愈伤组织诱导率/%	生根率/ %	愈伤组织诱导天数/d	生根天数/ d	平均一级根数/根	平均一级根长/cm	平均一级根粗/mm	根鲜质量/ g	根干质量/ g	根系效果指数	平均隶属函数值/U(X)	排序
T1	40.00	38.33	16.67	24.33	8.33	4.07	1.87	1.44	0.29	1.70	0.32	9
T2	51.67	50.00	18.00	25.00	8.11	4.03	1.77	1.59	0.35	1.64	0.36	8
T3	63.33	55.00	16.33	23.00	7.89	4.37	1.70	2.12	0.41	1.72	0.43	6
T4	65.00	53.33	17.67	26.00	9.11	4.57	1.83	2.10	0.40	2.08	0.43	7
T5	71.67	65.00	16.33	23.33	10.22	4.77	2.07	2.47	0.45	2.44	0.50	5
T6	66.67	60.00	14.67	22.00	11.33	5.50	2.30	2.24	0.41	3.11	0.50	4
T7	71.67	65.00	14.67	21.67	11.44	6.07	2.57	2.47	0.44	3.47	0.56	3
T8	78.33	70.00	13.67	18.67	12.33	6.37	2.63	2.79	0.51	3.93	0.63	2
T9	93.33	85.00	9.67	14.00	15.44	7.13	3.03	3.29	0.67	5.50	0.77	1
CK	23.33	21.67	25.00	32.33	4.89	3.10	1.57	1.21	0.24	0.76	0.23	10

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