庐山国家级自然保护区森林土壤细菌群落特征研究

doi:10.13466/j.cnki.lyzygl.2019.06.018

林业资源管理 ›› 2019›› Issue (6): 101-107.doi: 10.13466/j.cnki.lyzygl.2019.06.018

庐山国家级自然保护区森林土壤细菌群落特征研究

冯晓川¹(), 曹新光²(), 居文华³, 刘凯妹⁴

1. 国家林业和草原局调查规划设计院,北京 100714
2. 黄冈师范学院地理与旅游学院,湖北黄冈 438000
3. 内蒙古赤峰市巴林左旗林业和草原局,内蒙古赤峰 0254502
4. 湖北省团风中学,湖北黄冈 438000

收稿日期:2019-10-24 修回日期:2019-11-21 出版日期:2019-12-28 发布日期:2020-05-09
通讯作者: 曹新光
作者简介:冯晓川(1974-),女,河北人,工程师,硕士,主要从事信息工程、林业咨询等方面的工作。Email: fengxc1335@126.com
基金资助:
湖北省教育厅科研计划项目(青年项目);高级别培育项目(黄冈师范学院);科技部引智项目(GS20190157002)

A Study on Community Characteristics of Forest Soil Bacteria in Lushan National Nature Reserve

FENG Xiaochuan¹(), CAO Xinguang²(), JU Wenhua³, LIU kaimei⁴

1. Academy of Forest and Grassland Inventory and Planning,National Forestry and Grassland Administration,Beijing 100714
2. School of Geography and Tourism,Huanggang Normal University,Huanggang,Hubei 438000
3. Forestry and Grassland Bureau of BalinZuoqi,Chifeng,Inner Mongolia 025450
4. Hubei Tuanfeng Middle School,Huanggang,Hubei 438000

Received:2019-10-24 Revised:2019-11-21 Online:2019-12-28 Published:2020-05-09
Contact: CAO Xinguang

摘要/Abstract

摘要：

利用高通量测序技术分析了庐山国家级自然保护区不同海拔梯度典型森林土壤细菌群落特征。测序结果表明,酸杆菌门(53.84%)是庐山国家级自然保护区森林土壤平均相对丰度最高的门,其次为变形菌门(30.15%)和放线菌门(7.36%),而拟杆菌门及绿弯菌门所占比例较少(0.64%和0.28%);Ellin6513菌是第一大目分类水平细菌群落。从山麓到山顶空间位置相近的土壤细菌群落结构具有更大的相似性,不同海拔植被带土壤细菌群落多样性沿海拔梯度并没有表现出一定的变化趋势。在土壤化学指标中,与庐山森林土壤细菌群落相关性较大的是有机碳和总氮含量。

关键词: 土壤微生物, 高通量测序, 细菌群落结构, 海拔, 庐山

Abstract:

Soil microbe is ecologically important contributor to various functioning of forest ecosystems.Because of the elevation gradient,subtropical mountainous forests are a complex ecosystem where the nutrient availability and soil conditions vary with elevation levels.Therefore,the subtropical mountainous forests provide aunique opportunity to study the relationship between forest microbial communities and multiple environmental factors.In this study,we present a comprehensive analysis of soil bacterial community composition and diversity along six elevations representing six typical vegetation types in a subtropical mountainous forest at the Lushan,China.A total of 24 phyla,49 classes and 62 orders were confirmed with the method of high-throughput sequencing technology.At the phylum level,the dominant phyla were Acidobacteria(53.84%),Proteobacteria(30.15%)and Actinobacteria(7.36%);other phyla,such as Bacteroidetes and Chloroflexi(0.64% and 0.28%),were much less abundant.Ellin 6513 was the most abundant order in soil bacterial community.The composition and diversity of bacterial communities did not show a certain trend across an elevation gradient from low via medium to high.Among the soil chemical indexes,the organic carbon and total mitrogen confent are more relevent to the soil bacterial community in the Lushan forest.

Key words: soil microbe, high-throughput sequencing, bacterial community structure, elevation, Lushan

中图分类号:

冯晓川, 曹新光, 居文华, 刘凯妹. 庐山国家级自然保护区森林土壤细菌群落特征研究[J]. 林业资源管理, 2019,(6): 101-107.

FENG Xiaochuan, CAO Xinguang, JU Wenhua, LIU kaimei. A Study on Community Characteristics of Forest Soil Bacteria in Lushan National Nature Reserve[J]. FOREST RESOURCES WANAGEMENT, 2019,(6): 101-107.

图/表 6

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样品编号	取样位置	经纬度	海拔/m	土壤类型	森林植被类型	主要层优势植物
HHP-H1	好汉坡	29°35'57″N,115°59'15″E	570	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HHP-H2	好汉坡	29°35'45″N,115°39'11″E	680	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HHP-H3	好汉坡	29°35'33″N,115°59'09″E	776	山地黄壤	常绿阔叶林	微毛柃、大青、青冈
HLS-H4	黄龙寺	29°33'06″N,115°57'50″E	975	山地黄棕壤	常绿与落叶阔叶混交林	毛竹、小叶白辛树、樟树
DYS-H5	大月山	29°33'53″N,115°59'04″E	1250	山地棕壤	针阔混交林	短柄枹、花柏;微毛柃
WLF-H6	五老峰	29°32'55″N,116°00'49″E	1350	山地棕壤	针叶林	黄山松、杜娟

样品编号	枯枝落叶层厚度/cm	土壤总氮/(g/kg)	有机碳/(g/kg)	pH
HHP-H1	8	3.22	42.83	4.28
HHP-H2	6	4.72	68.55	4.26
HHP-H3	5	5.29	74.75	4.25
HLS-H4	4	5.43	78.29	4.54
DYS-H5	6	4.54	70.76	4.20
WLF-H6	3	4.78	73.21	3.88

样品编号	优化序列	OTUs数/个	Chao 1指数	Simpson指数	Shannon指数
HHP-H1	23031	342	375.454	0.972	6.266
HHP-H2	25314	343	352.282	0.980	6.520
HHP-H3	22071	346	355.692	0.973	6.331
HLS-H4	22947	366	382.912	0.985	6.856
DYS-H5	21144	325	340.857	0.976	6.433
WLF-H6	22206	360	384.972	0.981	6.630

庐山国家级自然保护区森林土壤细菌群落特征研究

A Study on Community Characteristics of Forest Soil Bacteria in Lushan National Nature Reserve

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