外源植物激素对白枪杆幼苗生物量分配的影响

doi:10.13466/j.cnki.lyzygl.2021.03.012

摘要/Abstract

摘要：

以白枪杆容器苗为材料,采用 L₉(3⁴) 正交实验方法,测定玉米素(ZT) 、3-吲哚乙酸(IAA) 和赤霉素(GA₃) 3 种外源植物激素不同组合处理下白枪杆幼苗的生物量,比较分析幼苗生物量及其分配与不同激素的关系,并采用标准主轴回归分析白枪杆幼苗各器官之间在不同外源植物激素处理下的异速生长关系。结果表明:不同外源植物激素中,ZT和IAA对幼苗生物量起主导作用,而GA₃作用不明显。不同处理间生物量及各器官生物量分配存在显著差异,4号处理(ZT 50mg/L,IAA 50mg/L,GA₃ 150mg/L)各器官生物量显著大于其他处理,但各器官生物量分配比不显著,9号处理(ZT 75 mg/L,IAA 150 mg/L,GA₃ 150mg/L)总生物量最小,但具有较高的茎生物量分配比。不同外源植物激素处理下各器官之间异速生长关系不同,但总体均呈等速生长,说明外源植物激素对白枪杆幼苗的异速生长影响不显著。不同外源植物激素作用下,白枪杆幼苗生物量显著改变,幼苗对环境中资源的分配无明显变化。

关键词: 白枪杆, 外源植物激素, 生物量分配, 异速生长

Abstract:

Taking Fraxinus malacophylla container seedlings as materials,L₉ (3 ⁴) orthogonal experiment method was used to determine the biomass of Fraxinus malacophylla seedlings under three exogenous plant hormones,zeatin (ZT),3-indoleacetic acid (IAA),and gibberellin (GA₃),the relationship between the biomass and its distribution of different seedlings and different hormones was compared and analyzed.The allometric growth relationship between different organs of Fraxinus malacophylla seedlings under different exogenous plant hormone treatments was analyzed using standard spindle regression.The results showed that among different exogenous plant hormones,ZT and IAA dominated the biomass of seedlings,and GA₃ had no significant effect;there was a significant difference in biomass and biomass allocation of different organs between different treatments.Treatment No.4 (ZT 50mg/L,IAA 50mg/L,GA₃ 150 mg/L) was significantly larger than other treatments,but the biomass allocation ratio of each organ was not significant.Treatment No.9 (ZT 75 mg/L,IAA 150 mg/L,GA3150 mg/L) had the smallest total biomass,but it had a higher stem biomass distribution ratio.The allogeneic growth relationship between different organs under different exogenous plant hormone treatments was different,but the overall growth was isokinetic,indicating that the effect of exogenous plant hormones on allometric growth of Fraxinus malacophylla seedlings was not significant.Under the action of different exogenous plant hormones,the biomass of Fraxinus malacophylla seedlings has changed significantly,and the distribution of resources in the environment by the seedlings has not changed significantly.

Key words: Fraxinus malacophylla, exogenous plant hormones, biomass allocation, allometry

中图分类号:

S792.99

段华超, 郑鑫华, 李燕燕, 叶澜, 井卉竹, 董琼. 外源植物激素对白枪杆幼苗生物量分配的影响[J]. 林业资源管理, 2021,(3): 76-83.

DUAN Huachao, ZHENG Xinhua, LI Yanyan, YE Lan, JING Huizhu, DONG Qiong. Effect of Exogenous Plant Hormones on Biomass Allocation of Fraxinus malacophylla Seedlings[J]. FOREST RESOURCES WANAGEMENT, 2021,(3): 76-83.

图/表 9

表1

表2

表3

表4

图1

表5

表6

白枪杆幼苗各器官间标准化主轴回归(SMA)分析

干重指标	处理	n	R²	P	SAM	95%CI	P_1.0
叶(y)—根(x)	1	18	0.852	0.009	0.5551	0.3330~0.9253	0.032
	2	18	0.394	0.182	0.6139	0.2405~1.5669	0.262
	3	18	0.534	0.099	1.3092	0.5630~3.0445	0.469
	4	18	0.339	0.226	0.7575	0.2873~1.9978	0.527
	5	18	0.074	0.602	1.3879	0.4620~4.1701	0.526
	6	18	0.433	0.155	1.4490	0.5815~3.6108	0.371
	7	18	0.345	0.220	1.2885	0.8711~2.0122	0.084
	8	18	0.324	0.238	0.8977	0.3377~2.3866	0.806
	9	18	0.053	0.660	0.6430	0.2121~1.9492	0.402
	CK	18	0.412	0.169	1.2150	0.4812~3.0674	0.636
叶(y)-茎(x)	1	18	0.664	0.048	1.3532	0.6480~2.8259	0.349
	2	18	0.593	0.073	0.3849	0.1733~0.8548	0.026
	3	18	0.814	0.014	1.5499	0.8781~2.7356	0.104
	4	18	0.307	0.254	1.3603	0.5069~3.6503	0.494
	5	18	0.132	0.478	1.2377	0.4228~3.6236	0.669
	6	18	0.579	0.079	1.2392	0.5516~2.7842	0.542
	7	18	0.206	0.366	1.3530	0.4784~3.8266	0.529
	8	18	0.284	0.277	0.5341	0.1965~2.7842	0.189
	9	18	0.130	0.483	0.7013	0.2393~2.0554	0.481
	CK	18	0.757	0.024	1.6971	0.8946~3.2194	0.088
茎(y)-根(x)	1	18	0.383	0.190	0.4102	0.1596~1.0540	0.061
	2	18	0.584	0.077	1.5950	0.7129~3.5685	0.208
	3	18	0.219	0.349	0.8447	0.3007~2.3731	0.721
	4	18	0.048	0.677	0.5569	0.1833~1.6920	0.273
	5	18	0.001	0.958	1.1214	0.3620~3.4742	0.830
	6	18	0.430	0.157	1.1693	0.4684~2.9190	0.699
	7	18	0.871	0.007	1.6914	1.0464~2.7339	0.038
	8	18	0.736	0.029	1.6809	0.8654~3.2648	0.102
	9	18	0.624	0.062	0.9169	0.4236~1.9846	0.791
	CK	18	0.359	0.208	0.7159	0.2747~1.8656	0.443
地上(y)-地下(x)	1	18	0.746	0.027	0.4798	0.2498~0.9218	0.034
	2	18	0.564	0.085	0.9423	0.4146~2.1414	0.866
	3	18	0.426	0.160	1.0651	0.4255~2.6660	0.876
	4	18	0.094	0.556	0.5896	0.1979~1.7566	0.310
	5	18	0.025	0.765	0.9994	0.3258~1.7566	0.999
	6	18	0.490	0.121	1.2550	0.5232~3.0106	0.556
	7	18	0.073	0.604	1.2615	0.4197~3.7918	0.652
	8	18	0.713	0.034	1.0906	0.5482~2.1697	0.762
	9	18	0.481	0.126	0.6872	0.2847~1.6592	0.347
	CK	18	0.421	0.163	1.0492	0.4179~2.6342	0.906

表6

表7

表8

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生物量	激素	平方和	自由度	均方	F统计量	显著性
总生物量	ZT	29.113	2	14.556	22.403	0.000
	IAA	10.799	2	5.399	8.310	0.001
	GA₃	4.980	2	2.490	3.832	0.029
	e	138.591	155	0.896
	Total	183.483	161
地上生物量	ZT	16.186	2	8.093	18.622	0.000
	IAA	6.160	2	3.080	7.087	0.002
	GA₃	3.121	2	1.560	3.590	0.035
	e	89.119	155	0.575
	Total	114.586	161
根生物量	ZT	2.107	2	1.053	18.115	0.000
	IAA	0.651	2	0.325	5.596	0.007
	GA₃	0.217	2	0.108	1.864	0.166
	e	10.803	155	0.070
	Total	13.778	161
茎生物量	ZT	3.175	2	1.587	13.576	0.000
	IAA	0.407	2	0.203	1.739	0.187
	GA₃	0.228	2	0.114	0.973	0.385
	e	18.389	155	0.119
	Total	22.199	161
叶生物量	ZT	5.892	2	2.946	14.987	0.000
	IAA	3.410	2	1.705	8.674	0.001
	GA₃	1.860	2	0.930	4.732	0.013
	e	43.529	155	0.281
	Total	54.691	161

处理	总	地上	根	茎	叶
1	4.85±0.93ab	3.68±0.58ab	1.17±0.39ab	1.38±0.18b	2.30±0.42a
2	4.48±0.73ab	3.52±0.60ab	0.96±0.16ab	1.33±0.40bc	2.19±0.23a
3	3.55±1.16bc	2.64±0.93bc	0.91±0.32b	1.21±0.35bc	1.43±0.62b
4	5.38±0.75a	4.19±0.63a	1.19±0.25a	1.84±0.25a	2.35±0.45a
5	3.71±0.79b	2.95±0.75bc	0.76±0.21bc	1.50±0.40ab	1.45±0.46b
6	3.85±0.85b	3.16±0.76b	0.69±0.12bc	1.38±0.33b	1.78±0.49ab
7	2.88±0.53c	2.36±0.55bc	0.52±0.09c	0.96±0.27c	1.40±0.60b
8	2.86±0.57c	2.25±0.47c	0.61±0.12c	1.01±0.33bc	1.24±0.20b
9	2.47±0.84c	1.98±0.64c	0.49±0.24c	0.96±0.50c	1.02±0.33b
CK	3.42±1.22bc	2.72±1.04bc	0.70±0.23bc	0.75±0.19c	1.97±0.86ab

测定项目	平方和	自由度	均方	F统计量	显著性
根分配比	0.152	9	0.013	3.189	0.006
茎分配比	0.140	9	0.016	4.729	0.000
叶分配比	0.145	9	0.016	3.400	0.003
地上分配比	0.219	9	0.009	2.578	0.028

干重指标			共同斜率	95%CI	t	p
叶(y)	—	根(x)	0.9560	0.6796~1.3375	11.694	0.200
叶(y)	—	茎(x)	1.2435	0.9188~1.6086	12.661	0.158
茎(y)	—	根(x)	1.1575	0.8253~1.5630	13.257	0.129
地上(y)	—	地下(x)	0.9149	0.6827~1.2063	6.616	0.620

指标			处理
指标			1	2	3	4	5	6	7	8	9	CK
叶(y)	—	根(x)	0.399ab	0.315ab	0.058b	0.427a	0.014b	0.072b	-0.166bc	-0.121bc	-0.342c	0.095b
叶(y)	—	茎(x)	0.527ab	0.474b	0.204bc	0.689a	0.342bc	0.395bc	0.073c	0.074c	-0.078c	0.094c
茎(y)	—	根(x)	0.190ab	0.081b	-0.006bc	0.337a	0.007b	-0.068bc	-0.372c	-0.267c	-0.460c	-0.338c
地上(y)	—	地下(x)	0.603a	0.519ab	0.339ab	0.678a	0.336b	0.332b	0.096c	0.144bc	-0.041c	0.250bc