Effect of Substrates and Exogenous Hormone on the Rooting of Air Layering of Berchemia floribunda(Wall.)Brongn.

doi:10.13466/j.cnki.lczyyj.2024.02.010

Abstract

Abstract:

Berchemia floribunda(Wall.)Brongn.is a traditional precious tree species in China with functions as a nectar source,ornamental and medicinal source,and has good ecological and economic benefits.In order to optimize the optimal conditions for B.floribunda air layering propagation and to improve the vegetative propagation technology,the L₉(3⁴)orthogonal matrix experiment was performed to explore the effects of different substrate ratios,exogenous hormone types,exogenous hormone concentration and girdle width on rooting of its air layering,subordination function method and correlation analysis method were used to comprehensively evaluate various rooting indices,and the mechanism of these four factors on rooting explored.The results showed that a suitable substrate and exogenous hormone ratio can promote the formation of callus,along with an increase in various rooting indices.Variance analysis and range analysis indicated that the efficiency of air layering was affected by the substrate ratio,exogenous hormone and girdle width,substrates ratio and exogenous hormone types were the two most important factors.Based on a comprehensive evaluation and correlation analysis,it is concluded that the most favorable treatment condition for B.floribunda rooting is:perlite+peat+vermiculite(1∶1∶1)as substrates,NAA is used as an exogenous hormone types with a concentration of 400 mg/L,and the girdle width is 10 mm.Under this treatment,the callus induction rate is as high as (93.33±2.36)%,and the rooting rate is as high as(85.00±4.08)%,(9.67±0.47)days to form a callus at the wound site,(14.00±0.82)days start to take root,average number of first order roots[(15.44±0.51)roots],average length of first order roots[(7.13±0.25)cm],average diameter of first order roots[(3.03±0.21)mm],fresh weight[(3.29±0.05)g],dry weight[(0.67±0.04)g],root effect index(5.50±0.06),average subordinate function value(0.77)were the highest.The results of this study provide theoretical references and data support for establishing a rapid propagation system of B.floribunda and promoting its cultivation.

Key words: Berchemia floribunda(Wall.)Brongn., air layering, rooting, substrates, exogenous hormone, orthogonal experiment

CLC Number:

S897

ZHAO Tian, WANG Yinchen, ZHOU Sixuan, ZENG Bing, YUAN Yang, DENG Mengqing, LIAO Yan, ZHANG Jinhua. Effect of Substrates and Exogenous Hormone on the Rooting of Air Layering of Berchemia floribunda(Wall.)Brongn.[J]. Forest and Grassland Resources Research, 2024, (2): 80-91.

Figures/Tables 11

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水平	因素
水平	基质配比 (A)	外源激素种类 (B)	外源激素浓度(C)/ (mg/L)	环剥宽度 (D)/ mm
1	草炭土+珍珠岩 (2∶1)	ABT 1	200	10
2	草炭+珍珠岩+蛭石 (3∶1∶1)	IBA	400	14
3	草炭土+珍珠岩+蛭石 (1∶1∶1)	NAA	600	18

处理	因素				因素水平组合
处理	基质配比 (A)	外源激素种类(B)	外源激素浓度(C)	环剥宽度 (D)	因素水平组合
T1	1	1	1	1	A₁B₁C₁D₁
T2	1	2	2	2	A₁B₂C₂D₂
T3	1	3	3	3	A₁B₃C₃D₃
T4	2	1	2	3	A₂B₁C₂D₃
T5	2	2	3	1	A₂B₂C₃D₁
T6	2	3	1	2	A₂B₃C₁D₂
T7	3	1	3	2	A₃B₁C₃D₂
T8	3	2	1	3	A₃B₂C₁D₃
T9	3	3	2	1	A₃B₃C₂D₁
CK

因素	自由度	愈伤组织诱导率		生根率		愈伤组织诱导天数		生根天数
因素	自由度	F	P	F	P	F	P	F	P
A	2	75.46	<0.001	54.86	<0.001	40.53	<0.001	64.26	<0.001
B	2	21.04	<0.001	18.03	<0.001	17.47	<0.001	27.00	<0.001
C	2	7.11	0.005	4.28	0.030	1.34	0.286	1.88	0.181
D	2	3.25	0.062	1.79	0.195	6.59	0.007	9.00	0.002
校正模型	8	26.71	<0.001	19.74	<0.001	16.48	<0.001	25.54	<0.001
R²		0.922		0.898		0.880		0.919

处理	平均一级根数/根	平均一级根长/cm	平均一级根粗/mm	根鲜质量/g	根干质量/g	根系效果指数
T1	8.33±0.34d	4.07±0.25e	1.87±0.06d	1.44±0.15d	0.29±0.01d	1.70±0.17e
T2	8.11±0.51d	4.03±0.15e	1.77±0.06d	1.59±0.49d	0.35±0.04c	1.64±0.17e
T3	7.89±0.51d	4.36±0.21de	1.70±0.10d	2.12±0.11c	0.41±0.05bc	1.72±0.06e
T4	9.11±0.38cd	4.57±0.12de	1.83±0.06d	2.10±0.08c	0.40±0.03c	2.08±0.12de
T5	10.22±0.51c	4.77±0.21d	2.07±0.06cd	2.47±0.10bc	0.45±0.02bc	2.44±0.16d
T6	11.33±0.67bc	5.50±0.20c	2.30±0.10c	2.24±0.10c	0.41±0.04bc	3.11±0.16c
T7	11.45±0.39bc	6.07±0.15bc	2.57±0.12b	2.47±0.12bc	0.44±0.04bc	3.47±0.20bc
T8	12.33±0.67b	6.37±0.21b	2.63±0.06b	2.79±0.20b	0.51±0.03b	3.93±0.34b
T9	15.44±0.51a	7.13±0.25a	3.03±0.21a	3.29±0.05a	0.67±0.04a	5.50±0.06a
CK	4.89±0.69e	3.10±0.26f	1.57±0.06d	1.21±0.16e	0.24±0.02e	0.76±0.13f

因素	自由度	平均一级根数		平均一级根长		平均一级根粗		根鲜质量		根干质量		根系效果指数
因素	自由度	F	P	F	P	F	P	F	P	F	P	F	P
A	2	215.79	<0.001	378.68	<0.001	196.68	<0.001	271.94	<0.001	71.21	<0.001	514.69	<0.001
B	2	33.78	<0.001	45.68	<0.001	16.87	<0.001	64.05	<0.001	28.75	<0.001	82.88	<0.001
C	2	10.36	<0.001	2.81	0.086	4.77	0.22	9.67	0.001	8.79	0.002	21.43	<0.001
D	2	21.84	<0.001	1.76	0.200	18.03	<0.001	21.82	<0.001	9.70	0.001	31.14	<0.001
校正模型	8	18.23	<0.001	107.24	<0.001	59.09	<0.001	91.87	<0.001	29.61	<0.001	162.53	<0.001
R²		0.969		0.979		0.963		0.976		0.929		0.986